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. 2021 May 19;7(21):eabg3217. doi: 10.1126/sciadv.abg3217

Fig. 4. LNP-Cas9 RNP/MSCM-NF/CXCL12α induces chemoattraction of leukemia cells and delivers Cas9 RNP to induce gene editing.

Fig. 4

(A) Loading profiles of CXCL12α on MSCM-NF. (B) CXCL12α release profiles from CXCL12α-loaded MSCM-NF for different loading amounts of CXCL12α (100, 200, or 300 ng of CXCL12α for 50 μg of MSCM-NF). The amount of CXCL12α released was proportional to the amount loaded. (C) Schematic of migration assay evaluating (i) cell migration by CXCL12α and (ii) the subsequent gene editing by LNP-Cas9 RNP delivery. (D) The percentage of THP-1 cells migrating to the lower chamber was measured after 4.5 hours of incubation. n = 2 to 3 independent studies. (E) Gene editing efficiency of LNP-Cas9 RNP/MSCM-NF/CXCL12α in migrated THP-1 cells. THP-1 cells that migrated to the lower chamber were collected, and a gene editing detection assay was performed after 2 days of treatment. L, 100-bp DNA ladder. Data are presented as means ± SD.