Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | fs(1)Yb | FlyBase | FBgn0000928 | |
| Gene (Drosophila melanogaster) | flamenco | FlyBase | FBgn0267704 | |
| Gene (Drosophila melanogaster) | Nup54 | FlyBase | FBgn0033737 | |
| Gene (Drosophila melanogaster) | Nup58 | FlyBase | FBgn0038722 | |
| Gene (Drosophila melanogaster) | Elys | FlyBase | FBgn0031052 | |
| Gene (Drosophila melanogaster) | Nup43 | FlyBase | FBgn0038609 | |
| Gene (Drosophila melanogaster) | Nup214 | FlyBase | FBgn0010660 | |
| Gene (Drosophila melanogaster) | Nup62 | FlyBase | FBgn0034118 | |
| Gene (Drosophila melanogaster) | Nup93-1 | FlyBase | FBgn0027537 | |
| Gene (Drosophila melanogaster) | Nup98-96 | FlyBase | FBgn0039120 | |
| Gene (Drosophila melanogaster) | Nxf1/Sbr | FlyBase | FBgn0003321 | |
| Gene (Drosophila melanogaster) | Nxt1 | FlyBase | FBgn0028411 | |
| Gene (Drosophila melanogaster) | lamin | FlyBase | FBgn0002525 | |
| Gene (Drosophila melanogaster) | Nup107 | FlyBase | FBgn0027868 | |
| Gene (Drosophila melanogaster) | Nup154 | FlyBase | FBgn0021761 | |
| Gene (Drosophila melanogaster) | Zuc | FlyBase | FBgn0261266 | |
| Gene (Drosophila melanogaster) | tj | FlyBase | FBgn0000964 | |
| Antibody | Anti-Piwi (Rabbit polyclonal) | DOI:10.1016/j.cell.2007.01.043 | IF(1:500) WB(1:5000) |
|
| Antibody | Anti-Yb (Mouse monoclonal) | DOI:10.1101/gad.1989510 | IF(1:500) WB(1:1000) |
|
| Antibody | Anti-Yb (Rabbit polyclonal) | DOI:10.1038/emboj.2011.308 | WB(1:1000) | |
| Antibody | Anti-tubulin (Rabbit polyclonal) | Abcam | Cat# ab18251, RRID:AB_2210057 |
WB(1:5000) |
| Antibody | Anti-HA (Rabbit monoclonal) | Cell Signaling Technology | Cat# 3724, RRID:AB_1549585 |
WB(1:1000) |
| Antibody | Anti-HA (Rabbit polyclonal) | Abcam | Cat# ab9110, RRID:AB_307019 |
WB(1:1000) |
| Antibody | Anti-FLAG (Mouse monoclonal) | Sigma | Cat# F1804, RRID:AB_262044 |
WB(1:1000) |
| Antibody | Anti-lamin (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat# adl67.10, RRID:AB_528336 |
IF(1:200) |
| Antibody | Anti-Nuclear Pore Complex Proteins (Mouse monoclonal) | Biolegend | Cat# 902907, RRID:AB_2565026 |
IF(1:500) |
| Antibody | Anti-Mouse IgG Alexa Fluor-488 (Goat polyclonal) | Thermo Fisher Scientific | Cat# A-11029, RRID:AB_2534088 |
IF(1:500) |
| Antibody | Anti-Rabbit IgG Alexa Fluor-647 (Goat polyclonal) | Thermo Fisher Scientific | Cat# A-21245, RRID:AB_2535813 |
IF(1:500) |
| Antibody | GFP-Booster Atto-488 (Alpaca monoclonal) | Chromotek | Cat# gba488-100, RRID:AB_2631386 |
IF(1:500) |
| Commercial assay or kit | IRDye 800CW Streptavidin | LI-COR | Cat# 926-32230 | WB(1:4000) |
| Commercial assay or kit | Streptavidin, Alexa Fluor 555 Conjugate | Thermo Fisher Scientific | Cat# S-21381 | IF(1:500) |
| Commercial assay or kit | Mouse monoclonal anti-FLAG M2 magnetic beads | Sigma-Aldrich | Cat# CatM8823, RRID:AB_2637089 |
|
| Commercial assay or kit | Dynabeads MyOne Streptavidin C1 | Thermo Fisher Scientific | Cat# 65001 | |
| Commercial assay or kit | Pierce IP Lysis Buffer-100 ml | Thermo Fisher Scientific | Cat# 87787 | |
| Commercial assay or kit | RIPA Lysis and Extraction Buffer | Thermo Fisher Scientific | Cat# 89901 | |
| Commercial assay or kit | Thermo Scientific Pierce anti-HA magnetic beads | Thermo Fisher Scientific | Cat# 88836 | |
| Commercial assay or kit | cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail | Sigma-Aldrich | Cat# 11836170001 | |
| Commercial assay or kit | RNasin Plus RNase Inhibitor | Promega | Cat# N2615 | |
| Commercial assay or kit | Effectene Transfection Reagent | Qiagen | Cat# 301427 | |
| Commercial assay or kit | Nucleofector Kit V | Lonza | Cat# VVCA-1003 | |
| Commercial assay or kit | DNase I, Amplification Grade | Thermo Fisher Scientific | Cat# 18068015 | |
| Commercial assay or kit | RNaseOUT Recombinant Ribonuclease Inhibitor | Thermo Fisher Scientific | Cat# 10777019 | |
| Commercial assay or kit | Magne HaloTag Beads,20% Slurry | Promega | Cat# G7282 | |
| Commercial assay or kit | Deoxynucleotide Solution Set (100 mM; 25 μmol each) | New England Biolabs | Cat# N0446S | |
| Commercial assay or kit | SuperScript III Reverse Transcriptase | Thermo Fisher Scientific | Cat# 18080085 | |
| Commercial assay or kit | Chloroform anhydrous 99+% | Sigma-Aldrich | Cat# 288306 | |
| Commercial assay or kit | TRIzol Reagent | Thermo Fisher Scientific | Cat# 15596026 | |
| Commercial assay or kit | Insulin solution human | Sigma-Aldrich | Cat# I9278 | |
| Commercial assay or kit | Fibronectin from human plasma 0.1% solution | Sigma-Aldrich | Cat# F0895 | |
| Commercial assay or kit | ProLong Diamond Antifade Mountant | Thermo Fisher Scientific | Cat# P36961 | |
| Commercial assay or kit | Fly Extract | Drosophila Genomics Resource Center | Cat# 1645670 | |
| Commercial assay or kit | SMARTer RNA Unique Dual Index Kit – 24U | Clontech | Cat# 634451 | |
| Commercial assay or kit | RiboPOOL 10 nM for Drosophila | Cambridge Bioscience | ||
| Commercial assay or kit | D-Biotin solution | Thermo Fisher Scientific | Cat# B20656 | |
| Commercial assay or kit | Shields and Sang M3 Insect Medium | Sigma | Cat# S3652 | |
| Commercial assay or kit | Library Quantification Kit | Kapa Biosystems | Cat# KK4873 | |
| Commercial assay or kit | ProTEV Plus | Promega | Cat# V6101 | |
| Commercial assay or kit | Paraformaldehyde, 16% w/v aq. soln., methanol free | Alfa Aesar | Cat# 043368.9M | |
| Commercial assay or kit | RNase A (affinity purified)1 mg/ml | Thermo Fisher Scientific | Cat# AM2271 | |
| Commercial assay or kit | NuPAGE 4–12%, Bis-Tris,1.5 mm, Mini Protein Gel, 10-well | Thermo Fisher Scientific | Cat# NP0335BOX | |
| Commercial assay or kit | Xfect Transfection Reagent | Takara Bio | Cat# 631318 | |
| Commercial assay or kit | Pierce Protein A/G Magnetic Beads | Thermo Fisher Scientific | Cat# 88802 | |
| Commercial assay or kit | Agencourt RNAClean XP beads | Beckman Coulter | Cat# A63987 | |
| Commercial assay or kit | NEBNext Ultra Directional RNA Library Prep Kit for Illumina | New England Biolabs | Cat# E7420L | |
| Commercial assay or kit | SMARTer Stranded RNA-Seq Kit | Takara Bio | Cat# 634839 | |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat# 74104 | |
| Commercial assay or kit | Cell Line Nucleofector kit V | Lonza | Cat# VVCA-1003 | |
| Commercial assay or kit | Nucleofector II device | Lonza | Cat# AAB-1001 | |
| Cell line (Drosophila melanogaster) | S2 cells | Thermo Fisher Scientific | Cat# R69007, RRID:CVCL_Z232 |
|
| Cell line (Drosophila melanogaster) | Ovarian somatic cells (OSCs) | DOI:10.1038/nature08501 | RRID:CVCL_IY73 | |
| Software, algorithm | Fiji | ImageJ | RRID:SCR_002285 | |
| Software, algorithm | Proteome Discoverer 2.1 | Thermo Fisher Scientific | RRID:SCR_014477 | |
| Software, algorithm | STAR | DOI:10.1093/bioinformatics/bts635 | RRID:SCR_015899 | |
| Software, algorithm | DEseq2 | DOI:10.1186/s13059-014-0550-8 | RRID:SCR_015687 | |
| Software, algorithm | Image Studio Lite | LI-COR | RRID:SCR_013715 |