Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Drosophila SRF (Mouse monoclonal) | Active Motif | Cat#:39093; RRID:AB_2793614 | lot# 03504001; IF(1:100) |
| Antibody | Anti-GFP (Rabbit polyclonal) | Invitrogen | Cat#:A11122; RRID:AB_221569 | IF(1:1000) |
| Antibody | Anti-Drosophila Patched; Apa 1, conc; (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat#:Apa1; RRID:AB_528441 | IF(1:100) |
| Antibody | Anti Drosophila Wingless; 4D4, conc; (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat#:4d4; RRID:AB_528512 | IF(1:100) |
| Antibody | Anti-Dachsous, 2828; (Mouse monoclonal) | Merkel et al., 2014 | IF(1:50) | |
| Chemical compound, drug | Grace’s insect medium | Sigma Aldrich | Cat#:G9771−10 × 1L | |
| Chemical compound, drug | Fetal Bovine Serum | Thermo-Fisher | Cat#:10270098 | |
| Chemical compound, drug | 20-Hydroxyecdysone | Sigma Aldrich | H5142 | |
| Chemical compound, drug | Penicillin-Streptomycin | Sigma Aldrich | P4333 | |
| Genetic reagent (D. melanogaster) |
Ecadherin-GFP; Ecad-GFP | Huang et al., 2009 | yw-; EcadGFP;; | |
| Genetic reagent (D. melanogaster) |
nub-Gal4 | Bloomington Drosophila Stock Center | Cat#:86108; RRID:BDSC_86108 | Combined with EcadGFP in this work |
| Genetic reagent (D. melanogaster) |
fat+dachsRNAi | Vienna Drosophila Resource Center | Cat#:9396; Cat#:12555 | Combined with Dcr2 on 1st chromosome and EcadGFP on 2nd in this work |
| Genetic reagent (D. melanogaster) |
pk30 | Bloomington Drosophila Stock Center | Cat#44229; RRID:BDSC_44229 | Combined with EcadGFP in this work |
| Genetic reagent (D. melanogaster) |
MyoVIRNAi | Vienna Drosophila Resource Center | Cat#37534 | Combined with EcadGFP in this work |
| Software, algorithm | TissueMiner | Etournay et al., 2016 |