Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | B6.C57BL/6/J: Ggta1+/+ | Obtained originally from Jackson Laboratory and maintained at IGC | N/A | |
| Strain, strain background (Mus musculus) | B6. Ggta1-/- | Obtained originally from Tearle et al., 1996 and maintained at IGC | N/A | |
| Strain, strain background (Mus musculus) | B6. Igha-/-Ggta1-/- | Singh et al., 2021 | N/A | |
| Strain, strain background (Mus musculus) | B6. Igh-J-/-Ggta1-/- | Yilmaz et al., 2014 | N/A | |
| Strain, strain background (Mus musculus) | B6. Rag2-/-Ggta1-/- | Singh et al., 2021 | N/A | |
| Strain, strain background (Mus musculus) | B6. Tcrb-/-Ggta1-/- | Yilmaz et al., 2014 | N/A | |
| Strain, strain background (Bifidobacterium bifidum) | Bifidobacterium bifidum BB-G90 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Bifidobacterium breve) | Bifidobacterium breve BB-G95 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Bifidobacterium infantis) | Bifidobacterium infantis BI-G191 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Bifidobacterium infantis) | Bifidobacterium infantis BI-G201 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Bifidobacterium lactis) | Bifidobacterium lactis BL-G101 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Bifidobacterium longum) | Bifidobacterium longum BL-G301 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Escherichia coli) | Escherichia coli K12 | ATCC | ATCC10798 | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Escherichia coli) | Escherichia coli O86:B7 | ATCC | ATCC12701 | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus acidophilus) | Lactobacillus acidophilus LA-G80 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus acidophilus) | Lactobacillus acidophilus LA-G436 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus bulgaricus) | Lactobacillus bulgaricus LB-G4058 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus bulgaricus) | Lactobacillus bulgaricus LB-G117 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus bulgaricus) | Lactobacillus bulgaricus LB-G40 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus casei) | Lactobacillus casei LC-G11 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus cremoris) | Lactobacillus cremoris LLC-G42 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus helveticus) | Lactobacillus helveticus LH-G444 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus casei) | Lactobacillus casei LC-G402 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus lactis) | Lactobacillus lactis LLL-G25 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus plantarum) | Lactobacillus plantarum Lp-G18 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus paracasei) | Lactobacillus paracasei LpC-G110 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus plantarum) | Lactobacillus plantarum Lp-G425 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus plantarum) | Lactobacillus plantarum Lp-G429 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus plantarum) | Lactobacillus plantarum Lp-G443 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus reuteri) | Lactobacillus reuteri Lr-G100 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus rhamnosus) | Lactobacillus rhamnosus Lr-G14 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus rhamnosus) | Lactobacillus rhamnosus Lr-G26 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Lactobacillus salivarus) | Lactobacillus salivarus LS-G60 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Strain, strain background (Streptococcus thermophilus) | Streptococcus thermophilus ST-G30 | BioGrowing Probiotics | N/A | See Materials and methods, Section Detection of αGal expression by probiotic bacteria |
| Antibody | Anti-Mouse Monoclonal Gr1 (Clone: RB6-8C5) | Bio-Rad | N/A | 300 µg in 200 µL PBS per mouse |
| Antibody | Anti-Mouse Monoclonal IgA (mA-6E1) | eBioscience, Thermo Fisher Scientific | Cat# 12-4204-82, RRID:AB_465917 | 1:100 (Flow cytometry) |
| Antibody | Goat Anti-Mouse Polyclonal IgA-HRP | Southern Biotech | Cat# 1040–05; RRID:AB_2714213 |
1:4000 (ELISA) |
| Antibody | Goat Anti-Mouse Polyclonal IgA-Unlabelled | Southern Biotech | Cat# 1040–01, RRID:AB_2314669 | 2 µg/mL (ELISA) |
| Antibody | Goat Anti-Mouse Polyclonal IgG, Human ads-HRP | Southern Biotech | Cat# 1030–05, RRID:AB_2619742 | 1:4000 (ELISA) |
| Antibody | Goat Anti-Mouse Polyclonal IgM, Human ads-HRP | Southern Biotech | Cat# 1020–05, RRID:AB_2794201 | 1:4000 (ELISA) |
| Antibody | Anti-α-gal Mouse Monoclonal IgG1 | Ding et al., 2008; Yilmaz et al., 2014 | N/A | Standard (ELISA). 0,5 μg/mL, 1:2 serial dilutions, 50 μL |
| Antibody | Anti-α-gal Mouse Monoclonal IgG2a | Yilmaz et al., 2014 | N/A | Standard (ELISA). 0,5 Originally o/mL, 1:3 serial dilutions, 50 μL |
| Antibody | Anti-α-gal Mouse Monoclonal IgG2b | Ding et al., 2008; Yilmaz et al., 2014 | N/A | Standard (ELISA). 0,5 μg/mL, 1:3 serial dilutions, 50 μL |
| Antibody | Anti-α-gal Mouse Monoclonal IgG3 | Ding et al., 2008; Yilmaz et al., 2014 | N/A | Standard (ELISA). 0,5 μg/mL, 1:2 serial dilution |
| Antibody | Anti-α-gal Mouse Monoclonal IgM | Yilmaz et al., 2014 | N/A | Standard (ELISA). 2 μg/mL, 1:2 serial dilutions, 50 μL |
| Antibody | Mouse IgA-Unlabelled | Southern Biotech | Cat# 0106–01, RRID:AB_2714214 | Standard 0.5 µg/mL(ELISA) |
| Commercial assay or kit | KAPA Mouse Genotyping Kit | KAPA Biosystems | Cat# KK7352 | |
| Commercial assay or kit | LEGEND MAX Mouse NGAL (Lipocalin-2) ELISA Kit | Biolegend | Cat# 443707 | |
| Commercial assay or kit | QIAamp Fast DNA Stool Mini Kit | Qiagen | Cat# 50951604 | |
| Chemical compound, drμg | Clodronate liposomes and control liposomes (PBS) | https://clodronateliposomes.com/ | SKU: CP-005–005 | 10 µL/g (in vivo depletion) |
| Chemical compound, drμg | Galα1-3Galβ1-4GlcNAc-BSA (14 atom spacer) | Dextra | Cat# NGP1334 | 5 µg/mL (ELISA) |
| Chemical compound, drμg | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), FITC conjμgate, lyophilized powder | Sigma-Aldrich | #L2895-1MG | 40 µg/mL (Flow cytometry) |
| Chemical compound, drμg | SYTO41 Blue Fluorescent Nucleic Acid Stain | Thermofisher Scientific | Cat# S11352 | 1:200 (Flow cytometry) |
| Software, algorithm | Greengenes | DeSantis et al., 2006 | http://greengenes.secondgenome.com/v0.13.8 | |
| Software, algorithm | QIIME | Caporaso et al., 2010 | http://qiime.org/v0.1.9.1 |