. 2021 May 18;15(3):034104. doi: 10.1063/5.0047185

TABLE II.

The effect of CPA loading time on re-expansion and hatching rates of vitrified–warmed blastocysts in the microfluidic approach and the manual method. Values with superscripts a–c are significantly different (mean ± SD, P < 0.05).

Groups		Re-expansion rate (%)	Hatching rate (%)
Manual CPA loading		97.40^a ± 1.53	44.40^a ± 5.69
On-chip CPA loading	6 min	86.61^b ± 3.25	22.73^b ± 1.90
	9 min	87.03^b ± 3.78	26.18^b ± 3.05
	12 min	96.07^a ± 1.88	38.80^a ± 1.02
	15 min	83.35^b ± 2.17	25.52^b ± 3.34
	18 min	84.06^b ± 3.08	17.01^c ± 0.85