Figure 4.

Pretreatment with 3-MA reverses the antiapoptotic effects of QUE on TBHP-treated NPCs. (a) Western blot analysis of the prosurvival (Bcl-2) and proapoptotic (Bax) proteins. (b) Quantification of the protein levels of Bcl-2 and Bax. (c) Apoptotic NPCs were stained with Annexin V PE/7-AAD double-fluorescence and analyzed by flow cytometry. (d) Quantification of the apoptotic rate based on the flow cytometry data. Data are presented as mean ± standard deviation from three independent experiments. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.