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. 2021 May 11;2021:6631562. doi: 10.1155/2021/6631562

Figure 5.

Figure 5

QUE regulates the ECM metabolic balance via autophagy in TBHP-treated NPCs. (a) Western blot analysis of the ECM components, collagen II and ACAN, and the degrading enzyme, MMP13. (b) Quantification of collagen II, ACAN, and MMP13 protein levels. (c, d) Representative images showing immunofluorescence staining for collagen II and MMP13 (collagen II: Alexa Fluor®488 labeled secondary antibody; MMP13: Alexa Fluor®594 labeled secondary antibody, original magnification ×200, scale bar: 75 μm). (e, f) Semiquantitative analysis of collagen II and MMP13 immunofluorescence staining. Data are presented as mean ± standard deviation from three independent experiments. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.