Cell Isolation buffer (PBS/2% FBS)

Reagent	Volume	Final concentration
Phosphate buffered saline (PBS), sterile, pH 7.4	490mL	-
Fetal bovine serum (FBS), heat-inactivated and sterile-filtered	10mL	2%
Total	500mL	-

Storage: Keep at 4°C until use. The cell isolation buffer can be stored for up to 7 days at 4°C, but we recommend making fresh buffer for every new sample.