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. 2021 Apr 6;296:100636. doi: 10.1016/j.jbc.2021.100636

Figure 5.

Figure 5

PIKfyve inhibition does not change the uptake of labeled K18 PFFs in neurons.A, representative images of primary hippocampal neurons treated with 1% DMSO, 100 nM or 1 μM YM-201636 before 16 h incubation with K18-AF488 and fixation (n = 3). B, imaging quantification of the number of K18-AF488 and FL Tau-AF488 positive vesicles inside neurons treated with 1% DMSO, 100 nM or 1 μM YM-201636 (n = 3). C, representative images of primary hippocampal neurons treated with either 1% DMSO or YM-201636 before incubation and imaging of K18-pHrodo uptake (scale bar: 50 μm). D, imaging quantification of K18-pHrodo in primary hippocampal neurons treated with the YM-201636 inhibitor in dose–response (n = 4). E, representative images of primary hippocampal neurons treated either with 1% DMSO or YM-201636 before fixation and staining for LAMP1. F, image quantification of the LysoTracker positive vesicles intensity after treatment with different concentrations of YM-201636 (n = 3).