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. 2021 Apr 6;296:100636. doi: 10.1016/j.jbc.2021.100636

Figure 6.

Figure 6

PIKfyve inhibition impairs the transport of K18 PFFs into lysosomes. A, representative images of primary hippocampal neurons treated with 1% DMSO or 1 μM YM-201636 before 16 h incubation with K18-AF488 and fixation and staining for LAMP1 (scale bar: 2 μm). B, image quantification of primary hippocampal neurons treated with 1% DMSO or 1 μM YM-201636 before a 16 h treatment with K18-AF488 or FL Tau-AF488 and fixation and staining for LAMP1 (Mann–Whitney test, ∗∗p < 0.05, n = 5 for LAMP1). C, image quantification of primary hippocampal neurons treated with 1% DMSO or 1 μM YM-201636 before 16 h treatment with K18-AF488 and fixation and staining for EEA1. D, representative images of mouse primary cultures treated with 1% DMSO, 100 nM or 1 μM YM-201636 before incubation with 20 μg/ml DQ-BSA (n = 3). E and F, representative images of mouse primary cultures treated with 1% DMSO, 100 nM or 1 μM YM-201636 and stained for cathepsin B and D respectively. G–I, imaging quantification of DQ-BSA puncta, cathepsin B and D inside neurons respectively (one-way ANOVA, nonparametric test, ∗<0.05, n = 3).