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. 2021 Jan 27;155(5):581–591. doi: 10.1007/s00418-020-01960-z

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© The Author(s) 2021

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Fig. 2 — Influence of ovarian steroid hormones and hCG on Ishikawa spheroid proliferation. Images show Ishikawa spheroids stained for the proliferation marker Ki-67 after 4 days of stimulation with estradiol/E2 (b/b’), progesterone/P4 (c/c’), medroxyprogesterone acetate/MPA (d/d’) or human choriogonadotropin/hCG (e/e’). a/a’ show the control without hormones. Arrows highlight the Ki-67 positive nuclei. Grey-scale pictures for Ki-67 in (a–e) and combination with nuclear staining (Hoechst; blue) in (a’–e’). Scale bar: 20 µm