Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 19;12:2951. doi: 10.1038/s41467-021-23217-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a N-glycan analysis of muscle fibronectin (Fn) isolated from control and LGMD2I and CMD patient cells. Blue box highlights the area of change at m/z 965.9 and m/z1111.4. b Quantification n = 3 averaged from three experimental replicates, each repeated with three technical replicates (*P < 0.05). c Co-immunoprecipitation of collagen released from purified fibronectin, normalised to healthy human controls (***P < 0.0001); three technical repeats repeated three times, box represents 5–95%, median centre line, whiskers = SEM. d Collagen and fibronectin binding quantitated via Biacore, before and after treatment with neuraminidase, an enzyme that specifically removes terminal sialic acid residues. e Schematic of the fibronectin rescue experiment. f Injection of human fibronectin into the single-cell stage of 1-dpf embryos. Fibronectin tagged with Alexa488 localises correctly to the myosepta in wild-type larvae, white arrows. Scale bar = 100 μm. Only fully sialylated fibronectin can rescue collagen deficits evident in fkrp^−/− larvae. fkrp^+/+ and fkrp^−/− larvae uninjected or injected, with either desialylated fibronectin or fully sialylated fibronectin, visualised for collagen localisation via z projections of the entire mediolateral extent of the myotome centred on the anal pore. Note also that fkrp^+/+ control fish, injected with sialylated fibronectin, exhibited a lower collagen deposition at the myoseptal boundaries. This lower collagen deposition is attributed to a previously described fibronectin–collagen feedback loop, scale bar = 100 μm. g Quantitation of the relative max intensity of collagen deposition at muscle basement membranes, 5 dpf, (***P < 0.0001), plotted points outside 95% confidence interval analysed by two-way ANOVA, Dunn’s multiple comparisons, from five repeats, box represents 5–95%, median centre line, whiskers = SEM. h Physiological rescue after fibronectin injection. Force transducer measurement of passive tension over stretch load % (n = 5 for each experimental condition) (***P < 0.0001), error bars = SEM.