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. 2021 May 19;12(6):511. doi: 10.1038/s41419-021-03790-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Heatmap showing changes in an array of fatty acids in SHEP cells with or without MYCN. Data are shown as relative changes in abundance compared to the PCDH vector. B Heatmap showing the expression profile of ferroptosis-associated metabolic genes. C Expression of MYCN induced iron metabolic genes analyzed by RT-qPCR, and SKP2 is positive control of MYCN targeted genes. D Western blot analysis of SHEP cells with or without MYCN expression using indicated antibodies. E, F TFRC and SLC40A1 expression in NB tumor tissues with or without MYCN amplification. Expression data were retrieved from GSE45547. Cellular iron load is detected with the colorimetric ferrozine-based assay in SHEP G and SK-N-BE2 H cells with or without MYCN, and values are normalized to protein concentration. Data are presented as mean ± SD of three replicates. Two-tailed unpaired t-tests were performed to calculate p values. **p < 0.01.