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. 2021 May 19;12(6):511. doi: 10.1038/s41419-021-03790-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Iron load and RT-qPCR analysis of SHEP cells with or without TFRC overexpression. B Iron load and western blot analysis of SK-N-BE2 cells with or without TFRC depletion. Cellular labile iron pool (LIP) was detected using the probe calcein acetoxymethyl ester (Cal-AM) in SHEP (C) and SK-N-BE2 (D) cells with or without TFRC. LIP was similarly detected in SHEP (E) and SK-N-BE2 (F) cells with or without MYCN. Data are presented as mean ± SD of three replicates. Two-tailed unpaired t-tests were performed to calculate p values. *p < 0.05, **p < 0.01.