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. 2021 May 19;12(6):511. doi: 10.1038/s41419-021-03790-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A RT-qPCR analysis of SK-N-BE2 cells with or without MYCN depletion. MYCN activation induced by 4-hydroxytamoxifen (4-HT) treatment promotes TFRC expression at both mRNA B and protein C levels. SKP2, DKK3 were used as positive and negative controls, respectively. D Amounts of cellular lipid peroxides measured by C11-BODIPY upon MYCN activation. E Primers designed for ChIP-qPCR analysis, and sequence variants for luciferase assays based on the TFRC promoter region. F ChIP-qPCR analysis of different binding sites of MYCN on the TFRC promoter. MDM2 is a positive control for MYCN binding. G Dual-luciferase assays based on (E). Signal values were normalized to no 4-HT treatment (day 0). Data are presented as mean ± SD of three replicates. Two-tailed unpaired t-tests were performed to calculate p values. **p < 0.01.