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. 2021 May 19;12:2941. doi: 10.1038/s41467-021-22719-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — 1 The cervical part of the fixed spinal cord was scanned and 2 produced strong myelin peaks in the diffraction patterns (white arrows). 3 Each 2D scan resulted in a projection depicting SAXS myelin peak intensity and 2D orientation of the myelinated axons. 4 Tensor-tomographic reconstruction of all projections provided 3D fiber orientations per voxel, here visualized by a vector map for a virtual section. Vectors are colored based on myelin levels in the corresponding voxel. 5 A probabilistic tractography algorithm enabled generating neuronal tracts for the specimen, an axial and a horizontal section of which are shown. Arrows point to anterior (orange) and posterior (white) horns.