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. 2021 May 19;12:2941. doi: 10.1038/s41467-021-22719-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Side projection of mean scattering intensity at myelin peak. b Same projection, with myelin-specific signal isolated. Color bars: photon-count. c, d 3D myelin maps at different myelin level (ML) thresholds for the region between dotted lines in a, b. ML units: scattered photons/voxel; ML = 4: highly myelinated areas in control mouse appear, no area in shiverer; ML = 0.1: white matter regions just appear in shiverer vs. almost entire brain for control. e Myelin levels for control (Ctrl-blue) and shiverer (Shi-orange) brains, for selected areas. ROI voxel numbers for control/shiverer: genu: 71/80, body anterior: 105/94, body posterior: 99/93, splenium: 136/63, internal capsule: 337/119. thalamus: 2740/210, cortex: 1018/403. White matter ROIs were eroded with a 3 × 3 × 3 box kernel to avoid partial volume effects. Box plots: notch → median, box → 25–75 percentiles, whiskers → ±2.7 standard deviations from mean, red crosses → outliers. Source data are provided as a Source Data file.