Table 1.

Experimental and reconstruction details for SAXS-TT scans.

	C57BL/6 brain (mouse)	Spinal cord (mouse)	V1 cortex (human)	CC splenium/body (human)	Rag2−/−(sh−/−) brains (mouse)
Beamline, synchrotron	cSAXS, SLS	LiX, NSLS-II	4-2, SSRL	cSAXS, SLS	LiX, NSLS-II
Tube diameter (mm)	10	3	20a	10	10
Beam energy (keV)	16.3	15.7	17	16.3	16.9
Beam size (μm2)	150 × 150	100 × 100	250 × 250	150 × 150	120 × 120
Motor step size (μm)	150	100	250	150	120
Field of view (pixels)	69 × 105	38 × 61	85 × 90	70 × 82	91 × 41
Field of view, size (mm2)	10.35 × 15.75	3.8 × 6.1	21 × 22.5a	10.5 × 12.3	10.92 × 4.92
No. of projections	267	60	151	254	181
Rotation axis tilt angles (°)	0, 10, 15, 20, 30, 40, 45, 50	0	0	0, 10, 20, 30, 40, 50	0
Nr of projections/tilt	67, 34, 32, 32, 30, 26, 24, 22	60	151	66, 56, 50, 44, 38	181
Exposure time/frame (ms)	125	300	120	80	100
Total exposure time (h)	67.1	11.6	38.5a	25.3	18.8
Total scan time (h)	87.2	17.4	61a	36.8	24.8
Doseb (Gy)	2.33 × 104	9.96 × 104	1.1 × 104	1.38 × 104	2.48 × 104
Reconstruction iterations	10,000	2,000	5,000	5,000	5,000

^a These values include scanning of two more specimens, not incorporated in the present study, that were scanned together with the V1 sample (in the same tube, next to one another, see Supplementary Fig. 1).

^bDose in $\mathrm{Gy}\left(=\frac{\mathrm{energy}\left(\mathrm{J}\right)}{\mathrm{mass}\left(\mathrm{kg}\right)}\right)$ was calculated as $\frac{\left(\mathrm{photon}\mathrm{flux}\right)\times \left(\mathrm{exposure}\mathrm{time}\right)\times \left(\mathrm{tissue}\mathrm{absorbtion}\right)\times \left(\mathrm{photon}\mathrm{energy}\right)}{\left(\mathrm{tissue}\mathrm{volume}\right)\times \left(\mathrm{tissue}\mathrm{density}\right)}$