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. 2021 May 19;12:2923. doi: 10.1038/s41467-021-23277-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a C22 cells were plated on transwells and transfected with Pdcd5 siRNAs. Mouse primary lung fibroblast cells (MLFs) were plated in 24-well plates a day before TGF-β₁ treatment. Transwells were transferred to Mlg-containing 24-well plates. After 24 h TGF-β₁ treatment, MLFs were counted using an automated cell counter (n = 3 in each group); ****p < 0.0001. b MLFs were cultured in C22 conditioned medium (CM). The levels of indicated genes were analyzed by qRT-PCR (n = 3 in each group) *p = 0.0265; ***p = 0.0002; ****p < 0.0001; n.s. not significant. Error bars represent mean ± s.e.m., statistical analysis was performed with one-way ANOVA followed by Tukey’s test (a, b). c C22, RLE-6TN, and Mlg cells were treated with TGF-β₁ for 24 h. Enriched media and cell lysates were used for immunoblotting with indicated antibodies. Representative blots from three independent experiments are shown. d IHC with indicated antibodies was performed in the lung tissue from Pdcd5^f/f and Ccsp-Pdcd5^d/d mice. Scale bars = 100 μm. Representative images from three mice in each group are shown. Source data are provided in the Source Data file.