Fig. 2. Behavioral results following optogenetic inhibition of the aRSC during TFC (Mean/SEM).

A–D Inhibition of the aRSC during the CS–UCS pairings. E–H aRSC inhibition during ITI of training. Design schematic depicted directly above figures. Green overlay in schematic indicates temporal placement of optogenetic inhibition. No group differences were demonstrated during training. UCS deliveries are marked by dashed lines (A, E). When inhibition of the aRSC occurred in the CS of acquisition but not the ITI, ArchT animals froze less during the CS during the CS test (B, F). No differences were seen when tested in the acquisition context (C, G), indicating that inhibition of the aRSC during acquisition selectively impacts CS freezing. D, H Representative images of virus expression along the sagittal plane (×4 magnification). Asterisks indicate p < 0.05.