Fig. 3. Behavioral results following optogenetic inhibition of the pRSC during TFC (Mean/SEM).

A–D aRSC inhibition during CS–UCS pairings. E–H Inhibition during ITI of training. Experimental design schematic depicted directly above figures. Green overlay in schematic indicates temporal placement of optogenetic inhibition. UCS deliveries are marked by dashed lines (A, E). No group differences were demonstrated during training (A, E) or during the CS test (B, F). Animals froze less to the acquisition context when their pRSC was inhibited during the training CS (C) but not when it was inhibited during the training ITI (G). Thus, pRSC inhibition during acquisition selectively impacts context freezing. D, H Representative images of virus expression along the sagittal plane (×4 magnification). See text for more details. Asterisks indicate p < 0.05.