Table 2.
The main primers used in this study
| Primers | Sequence |
|---|---|
| P1 | CATGCGGCCGCTTAGCTTGCAGTGGGCTTACATGG |
| P2 | CGGTCCGGAAACCCCAGAGTCCCGCTCAG |
| P3 | CGGTCCGGAGCAGTCATGTCGTGCTAATGTGTAAAAC |
| P4 | CCGCTCGAGCATTGTCAACAACAAGACCCATCAT |
| P5 | CCGCTCGAGGGGATAACGCAGGAAAGAACATG |
| P6 | GCGGGCCCCCTTAACGTGAGTTTTCGTTCCACT |
| P7 | CATGCGGCCGCCCAGCTCATAGACCGTATCCAAAG |
| P8 | TCCCCCGGGTTTCCTGCGTTATCCCCTGATT |
| P9 | CTTGAGCTGTTGACAATTAATCATCGTGTGGTACCATACTAGTGGATCCTTCCTACCC |
| P10 | GGGTAGGAAGGATCCACTAGTATGGTACCACACGATGATTAATTGTCAACAGCTCAAGGGCC |
| P11 | GATCCTTCCTAGCTAGCTTGGTTGGCGCGCCAATACTTAAGCCC |
| P12 | GGGCTTAAGTATTGGCGCGCCAACCAAGCTAGCTAGGAAG |
| P13 | CATGCGGCCGCCAGTGGAACGAAAACTCACGTTAAG |
| P14 | GCTCCTAGGAGCTCATAGACCGTATCCAAAGCAT |
| P15 | GCTCTAGATAGCTTGCAGTGGGCTTACATGG |
| P16 | ATTCTCGAGTCACGTAGCGATAGCGGAGTGT |
| P17 | ATTCTCGAGCTGGCACGCATAGCCAAGCTAG |
| P18 | CATGCGGCCGCCATTGTCAACAACAAGACCCATCAT |
| P19 | GCTCTAGAGCCTGGGGTGCCTAATGAGT |
| P20 | CATGCCATGGTACAACGTCGTGACTGGGAAAAC |
| ldhsg-F | GTGGATCCCAGCATGTAGCGGAATCGAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| ldh-U-F | CCGCTCGAGTGCTTCCAGACGGTTTCATC |
| ldh-U-R | CACCTTGCGATCATCGACATAAGGGCTCCACTTCCTACGG |
| ldh-D-F | CCGTAGGAAGTGGAGCCCTTATGTCGATGATCGCAAGGTG |
| ldh-D-R | TTCCTAGCTAGCATCGGCCAAGGTCAAAGTG |
| eutDsg-F | CGGGATCCGTCGCCGTTGTGGACCATCAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| eutD-U-F | CCGCTCGAGACAAATTCATGGGAGGTGCC |
| eutD-U-R | AAGCAAGGCAAGCACGTTGGCGCAAGAAGATGCCAGACT |
| eutD-D-F | AGTCTGGCATCTTCTTGCGCCAACGTGCTTGCCTTGCTT |
| eutD-D-R | GCTCTAGAGCGTAGATTCCTGCTGGACC |
| gabPsg-F | GTGGATCCGTGGAGGCATTGATCACCACGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| sgRNA-R | CACGACAGGTTTCCCGACTG |
| gabP-F | GTGGTACCATACTAGTGGATCCGTGGAGGCATTGATC |
| gabP-U-F | CAGTCGGGAAACCTGTCGTGTGCCACTTTCACCGACTTGG |
| gabP-U-R | CACAAACGCCAGGGAGTACAGCAGGGATACTTCGGCGATG |
| gabP-D-F | CATCGCCGAAGTATCCCTGCTGTACTCCCTGGCGTTTGTG |
| gabP-D-R | GCCAACCAAGCTAGCTAGGAACCGTGATGCTGCCTCTTCTAG |
| glnA1sg-F | CGTGGATCCCGTGGAGCATGGTGTTGAAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| glnA1-U-F | ATTCTCGAGACAATAGCAATAACCCAGGAAACAC |
| glnA1-U-R | AGGAGTTCAGGGTTGCGTTGCGAGGTCTGGCAGGAGATTC |
| glnA1-D-F | GAATCTCCTGCCAGACCTCGCAACGCAACCCTGAACTCCT |
| glnA1-D-R | TGCTCTAGACATCTGAACTGATCGGCATCTAG |
| glnA2sg-F | CGTGGATCCGAAATATCCGCCGTTGTCAGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| glnA2sg-R | GGATTTGTTGTGGGGCTTGTCACGACAGGTTTCCCGACTG |
| glnA2-F | CGTGGATCCGAAATATCCGCCGTTGTCAG |
| glnA2-U-F | CAGTCGGGAAACCTGTCGTGACAAGCCCCACAACAAATCC |
| glnA2-U-R | CCAGATCCATCGACATTCCATTCGTGTTCCTACCTACCGTTTG |
| glnA2-D-F | CAAACGGTAGGTAGGAACACGAATGGAATGTCGATGGATCTGG |
| glnA2-D-R | TTCCTAGCTAGCCTCATCGGAGCAGGAGTAAGC |
| gdhA1sg-F | CGTGGATCCGCAAACGCCTAGGATGTACAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| gdhA1-F | GTGGTACCATACTAGTGGATCCGCAAACGCCTAGGATG |
| gdhA1-U-F | CAGTCGGGAAACCTGTCGTGTGATGCGGTAGCGGTTCCTTTG |
| gdhA1-U-R | GTTCACATCAACCGGCTTGTCATAC |
| gdhA1-D-F | GTATGACAAGCCGGTTGATGTGAACGGGGAAGAATTAGGCAGGCATC |
| gdhA1-D-R | AAGCTAGACCCGGGCTTAAGCTCACGAAGTAAACGCAGCCGTAG |
| alaTsg-F | CGTGGATCCGGTTGCCAGGCTGATGTGCTGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| alaTsg-R | ATTCTCGAGCACGACAGGTTTCCCGACTG |
| alaT-U-F | ATTCTCGAGCGGGGTAATGCCATAACGAG |
| alaT-U-R | GAATAGCGTGCTGAGCTGGGCGGAATAATGCCTTTGGAGGT |
| alaT-D-F | ACCTCCAAAGGCATTATTCCGCCCAGCTCAGCACGCTATTC |
| alaT-D-R | TTCCTAGCTAGCGACGCAGCAAGACCTGACATAC |
| argRsg-F | CGTGGATCCTCGCGGGCGATGCTATCTACGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| argRsg-R | CAAAGCCAATCATGTAGGAGTTGCACGACAGGTTTCCCGACTG |
| argR-F | TGGTACCATACTAGTGGATCCTCGCGGGCGATGCTATCTAC |
| argR-U-F | CAGTCGGGAAACCTGTCGTGCAACTCCTACATGATTGGCTTTG |
| argR-U-R | CGAGAACGAAAACGGTGTCATAGTTGTACCTGGCTGGTGACTT |
| argR-D-F | AAGTCACCAGCCAGGTACAACTATGACACCGTTTTCGTTCTCG |
| argR-D-R | TGGCGCGCCAACCAAGCTAGCAACCTGGTCGTTGCCCTTAC |
| gabsg-F | CGGGATCCCCAGTAATCAACCCCAGCGAGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| gab-U-F | CCGCTCGAGACGAGTTCGCTGATTTGGATG |
| gab-U-R | CAAGTCGGTGAAAGTGGCAACATGGTGAGGTTGGTCCGTC |
| gab-D-F | GACGGACCAACCTCACCATGTTGCCACTTTCACCGACTTG |
| gab-D-R | GCTCTAGAGGTGCCTAAAACAAAGAATCCAAG |
| aceABsg-F | CGTGGATCCGGAAATCCTCGTACGCCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| aceABsg-R | GCCTCATCGGTGTCGTTGTAACACGACAGGTTTCCCGACTG |
| aceAB-F | TGGTACCATACTAGTGGATCCGGAAATCCTCGTACG |
| aceAB-U-F | CAGTCGGGAAACCTGTCGTGTTACAACGACACCGATGAGGC |
| aceAB-U-R | GTATCCGAGGATGGACTGGCAGGAACTCGGCGCAATGGGCT |
| aceAB-D-F | GGAACTCGGCGCAATGGGCT |
| aceAB-D-R | TGGCGCGCCAACCAAGCTAGCCCAGGGTTCGCTACGGAATC |
| poxBsg-F | GTGGATCCGGTCACCGGATACTTCACCGGTTTTAGAGCTAGAAATAGCAAGTTAAAAT |
| poxB-F | GTGGTACCATACTAGTGGATCCGGTCACCGGATACTTC |
| poxB-U-F | CAGTCGGGAAACCTGTCGTGGTTGCACTGCATGATCGGTT |
| poxB-U-R | CGCTGAAGGCTGTGGTGTTT |
| poxB-D-F | AAACACCACAGCCTTCAGCGTCGCAGTAACCAGAGCATTCC |
| poxB-D-R | AAGCTAGACCCGGGCTTAAGGTTTTCGAGGCGACCAGACAG |
| speC-F | ATTCTCGAGTCACATGTTCTTTCCTGCGTTATC |
| speC-R | AAGCAAGGCAAGCACGTTGTTACTTCAACACATAACCGTACAACCG |
| gdhA-F | AGTCTGGCATCTTCTTGCGCGACGTTTGAGCTGTTGACAATTAATC |
| gdhA-R | GAGCTCGAATTCTTAAATCACACC |
| gadB-F | CATCGCCGAAGTATCCCTGCATTTTGGGGAAGAATTAGGCAG |
| gadB-R | CGCCAGGGAGTACATCAGGTATGTTTAAAGCTGTTCTGTT |
Italics is the site of restriction endonuclease or homologous sequence of vector pCCG1/pCCG2 for recombinant plasmid construction using one-step cloning ligation