Fig. 2.

Effects of SPJs on the expression levels of Collagen I, Collagen III, Collagen IV, Fibronectin and α-SMA in the renal tissue of aging rats. The mRNA expression level of Collagen I(A), Collagen III (B) and Collagen IV(C) were shown. (D), (E) Representative results of Western blot and quantitative analysis with antibodies against Collagen I, Collagen III and Collagen IV. β-actin was used as an internal control for normalization of protein loading. SPJs affected protein expression levels of Fibronectin (F) and α-SMA (G) in the renal tissue of aging rats as detected by immunohistochemistry (400×). (H) The quantitative analysis on protein expression levels (positive areas) of fibronectin and α-SMA in the kidneys. (K) The relative mRNA expression levels (folds) of Fibronectin and α-SMA in the kidneys. All the experiments were performed in triplicate. Data in (E), (H) and (K) are expressed as mean ± SD, ∗p < 0.05, n = 6.