Fig. 3.

Effects of SPJs on the expression levels of MMP2, MMP9, TIMP1 and TIMP2 in the renal tissue of aging rats. The mRNA expression levels of MMP2 (A), MMP9 (B), TIMP1(C) and TIMP2 (D). (E) Effects of SPJs on the protein expression levels of MMP2, MMP9, TIMP1 and TIMP2 as detected with their specific antibodies via Western blot. Antibody against β-actin was used as an internal control for normalization of protein loading; (F) The quantitative relative protein expression levels of MMP2, MMP9, TIMP1 and TIMP2 based on Western blot. All the experiments were performed in triplicate. Data in (A-D) and (F) were expressed as mean ± SD, ∗p < 0.05, n = 6.