Fig. 5.

Effects of SPJs on the expression levels of inflammatory factors and NF-κB signaling in the renal tissue of aging rats. (A) The mRNA expression levels of TNF-α, IL-1β and IL-6 detected by real time PCR. (B) Representative results of Western blot and quantitative analysis with antibodies against TNF-α, IL-1β and IL-6; β-actin was used as an internal control for normalization of protein loading; (C) Representative results of Western blot and quantitative analysis with antibodies against NF-κB, IκB, and the phosphorated NF-κB, IκB, β-actin was used as the internal control for normalization of loading control. All the experiments were performed in triplicate. Data in (A-C) were expressed as mean ± SD, ∗p < 0.05, n = 6.