Table 1.
The primer sequences used for real-time PCR
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Nrf2 | gacctaaagcacagccaacacat | ctcaatcggcttgaatgtttgtc |
| TGF-β1 | tgcgcttgcagagattaaaa | agccctgtattccgtctcct |
| Smad2 | aacccgaatgtgcaccataagaa | gcgagtctttgatgggtttacga |
| Smad3 | ccccagagcaatattccaga | tgtgaagcgtggaatgtctc |
| Smad4 | aaggcctagcaccaccttag | agccttaaactctgacctgt |
| HO-1 | tgtcccaggatttgtccgag | actgggttctgcttgtttcgct |
| NQO1 | ggggacatgaacgtcattctct | agtggtgactcctcccagacag |
| TIMP1 | gggcttcaccaagaccta | gaagaaagatgggagtggg |
| TIMP2 | ccaaagcggtcagtgaga | tggtgcccgttgatgttc |
| MMP2 | acctggatgccgtcgtggac | tgtggcagcaccagggcagc |
| MMP9 | cgctgggcttagatcattcc | ttgtcggcgataaggaagg |
| Fibronectin | gactcgctttgacttcaccac | tccttcctcgctcagttcgt |
| Collagen I | actcagccgtctgtgcctca | ggaggcctcggtggacatta |
| Collagen III | aagggcagggaacaactgat | gtgaagcagggtgagaagaaac |
| Collagen IV | ccgggatttactggaccacc | cccttgctctcccttgtca |
| TNF-α | tgctctgtgaggcgactgg | gggctctgaggagtagacgataaag |
| IL-1β | ggaaacagcaatggtcggg | gacttggcagaggacaaaggc |
| IL-6 | caacttccaatgctctcctaatgg | tgccgagtagacctcatagtgacc |
| GAPDH | aactttggcattgtggaagg | acacattgggggtaggaaca |