Skip to main content
. 2021 May 11;24(1):509. doi: 10.3892/mmr.2021.12148

Figure 6.

Figure 6.

Effects of THCA on the expression of HO-1/NQO1 and the activation of Nrf2 in HaCaT cells. (A) Levels of HO-1 and NQO1 expression and Nrf2 phosphorylation were determined using western blot analysis. HaCaT cells were treated with THCA for 16 h to detect the levels of HO-1 and NQO1 expression. Cells were also treated with THCA for 1 h to detect the levels of Nrf2 activation. (B) Quantitative analysis of HO-1, NQO1 and p-Nrf2 was performed using ImageJ. (C) The level of Nrf2 nuclear translocation was determined using western blot analysis. HaCaT cells were treated with THCA for 1 h. Then, nuclear and cytosolic fractions were obtained. (D) Quantitative analysis of Nrf2 expression was performed using ImageJ. Data are expressed as the means ± standard deviation. (E) Levels of Nr2 nuclear translocation were determined using immunocytochemistry. Scale bar, 20 µm. *P<0.05 vs. negative control group. THCA, 3,4,5-trihydroxycinnamic acid; HO-1, heme oxygenase-1; NQO1, NAD(P)H quinone dehydrogenase 1; Nrf2, nuclear factor erythroid 2-related factor 2; DAPI, 4′,6-diamidino-2-phenylindole.