Figure 2.

Representative photomicrographs of myocardium from the left ventricles of both former-preterm lambs at (A) 2-month TEA and (B) 5-month TEA, and term lambs at (C) 2-month TEA and (D) 5-month TEA, showing negligible levels of apoptotic cardiomyocyte nuclei (green) as assessed by the TUNEL assay. (E) Rat breast tumour sections were used as a positive control. Sections were counterstained with DAPI (blue). Scale bars = 200 μm. DAPI, 4,6-diamidino-2-phenylindole hydrochloride; TEA, term-equivalent age; TUNEL, terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling.