FIGURE 6.

MiR‐181a‐5p regulates the function of NK cells. Splenocytes of the young mice (a) or NKL cells (b) were transfected with NC or miR‐181a‐5p antagomir, and stimulated by YAC‐1 or K562 cells, respectively. Representative flow cytometry plots (left) and statistical results (right) showing the IFN‐γ⁺ cells in gated NK1.1⁺CD3⁻ NK cells or NKL cells. (c) Splenocytes of the young mice were transfected with NC or miR‐181a‐5p antagomir, and stimulated by YAC‐1 cells. Representative flow cytometry plots (above) and statistical results (below) showing the frequency of CD107a⁺ cells in gated NK1.1⁺CD3⁻ NK cells. (d) NKL cells (left) or splenocytes of the young mice (right) were transfected with NC or miR‐181a‐5p antagomir. Lysis of target cells was shown in the summary graph (K562 cells (left); YAC‐1 cells (right)). (e) Lysis of YAC‐1 cells was shown. Splenocytes from young or aged mice were used as effectors. (f) NK cells were sorted from the PBMC of young and elderly people. The transcript level of miR‐181a‐5p, miR‐223‐3p, or miR‐126a‐5p was detected by qTR‐PCR. Data represent three independent experiments (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001