FIGURE 4.

Telomerase deficiency increases DNA damage and senescence and reduces proliferation in Club cells following HDM‐induced allergy. a, Representative images of proximal airways showing immunostainings for SCGB1A1 (blue) and H2AX (brown; red arrowheads indicate double SCGB1A1⁺‐H2AX⁺ Club cells) (left), SCGB1A1 (blue), and p21 (brown; black arrowheads indicate double SCGB1A1⁺‐p21⁺ cells) (center), and SCGB1A1 (blue) and Ki67 (purple; green arrowheads indicate double SCGB1A1⁺‐Ki67⁺ cells) (right) in lung sections from Tert ^+/+ and G3 Tert ^−/− mice. Quantification of SCGB1A1⁺‐H2AX⁺ (b), SCGB1A1⁺‐p21⁺ (c) and SCGB1A1⁺‐Ki67⁺ (d) Club cells per epithelium length in Tert ^+/+ and G3 Tert ^−/− mice. Quantifications in lung sections were performed in 4 different bronchi in a random way. Data are expressed as mean ±SEM. **p < 0.01; ***p < 0.001 (Dunn‐Sidak multiple comparison test). The number of mice is indicated in each case