Fig. 2.

EcMltG favours ongoing glycan strand polymerisation for LT activity. Levels of crosslinked muropeptides (TP: blue columns) and LT product (LT: grey columns) obtained after cellosyl-digestion of the PG synthesised by EcPBP1A, EcPBP1B and/or EcMltG, from E. coli-type lipid II. Values are the mean ± variation of two independent experiments. EcMltG has a lytic transglycosylase activity in the presence of EcPBP1A or EcPBP1B. EcMltG reduces the TPase activity of EcPBP1A and EcPBP1B. EcPBP1B S510A and BsMltG E218Q, catalytically inactive versions (controls); Amp, Ampicillin. **, P < 0.01; *, P < 0.05 (T-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)