Table 5.
Details of Applied Primary and Secondary Antibodies
| Primary antibody | Species | Company | Working concentration |
|---|---|---|---|
| Anti-AMPKα (#2532) | rabbit | Cell Signaling Technology, Danvers, Massachusetts | 1 μg/mL (WB) |
| Anti-α-TUBULIN (#2125) | rabbit | Cell Signaling Technology, Danvers, Massachusetts | 1 μg/mL (WB) |
| Anti-β-ACTIN (#4967) | rabbit | Cell Signaling Technology, Danvers, Massachusetts | 1 μg/mL (WB) |
| Anti-CASPASE-1 (#2225) | rabbit | Cell Signaling Technology, Danvers, Massachusetts | 1:100 (IHC); 1:1000 (WB)a |
| Anti-mouse IgA α-chain (PAB9360) | rabbit | Abnova, Taipei, Taiwan | 0.5 μg/mL (ELISA) |
| Anti-mouse Ig light chain κ (407201) | rat | BioLegend, San Diego, CA | 1 μg/mL (ELISA) |
| Anti-mouse Ig light chain λ (407302) | rat | BioLegend, San Diego, CA | 1 μg/mL (ELISA) |
| Anti-human KLF4 (AF3640) | goat | R&D Systems, Minneapolis, Minnesota | 4 μg/mL (IHC); 1 μg/mL (WB) |
| Anti-murin KLF4 (AF3158) | goat | R&D Systems, Minneapolis, Minnesota | 3.3 μg/mL (IHC) |
| Anti-MUC2 (clone H-300) | rabbit | Santa Cruz Biotechnologies, Dallas, Texas | 2 μg/mL (IF) |
| Anti-Muc5AC (clone 45M1) | mouse | Bioss, Woburn, Massachusetts | 2 μg/mL (IHC); 1 μg/mL (ELISA) |
| Anti-pAMPKα (Thr 172, #2535) | rabbit | Cell Signaling Technology, Danvers, Massachusetts | 1 μg/mL (WB) |
| Anti-p32 (clone EPR8871) | rabbit | Abcam, Cambridge, United Kingdom | 2 μg/mL (IHC); 0.4 μg/mL (WB) |
| Anti-p32 (clone 60.11) | mouse | Abcam, Cambridge, United Kingdom | 1 μg/mL (WB) |
| Anti-p32 exon 6 | rabbit | kindly provided by Berhane Ghebrehiwet | 5 μg/mL (IHC) |
| Anti-TOMM22 (#WH0056993M1) | rabbit | Sigma-Aldrich, St Louis, Missouri | 2.5 μg/mL (IHC) |
| Secondary antibodies/labeled polymers | Company | Working concentration | |
|---|---|---|---|
| Alexa Fluor 594 nm goat anti rabbit | Thermo Fisher Scientific, Waltham, Massachusetts | 8 μg/mL (IF) | |
| Anti-mouse IgG HRP | Cell Signaling Technology, Danvers, Massachusetts | 1:1000 (ELISA); 1:4000 (WB)a | |
| Anti-rabbit IgG HRP | Cell Signaling Technology, Danvers, Massachusetts | 1:2000 (ELISA); 1:4000 (WB)a |
|
| Anti-goat IgG HRP | Agilent, Santa Clara, California | 1 μg/mL (IHC); 0.5 μg/mL (WB) | |
| EnVision anti-rabbit HRP system | Agilent, Santa Clara, California | Not applicable | |
| EnVision anti-mouse HRP system | Agilent, Santa Clara, California | Not applicable |
ELISA, enzyme-linked immunosorbent assay; IHC, immunohistochemistry; WB, Western blot.
a
If stock concentration was not provided by the manufacturer, dilution from stock solution was listed.