Figure 7.

Histone variant macroH2A1.2 promotes KDM5A recruitment to DNA damage sites. (A) macroH2A1 promotes KDM5A damage localization. Analysis of GFP-KDM5A damage recruitment in macroH2A1-KO U2OS cells was performed as in Fig. 3 F. Scale bars, 5 µm. (B) Quantification of A. n ≥ 10 cells. Error bars represent SEM. (C) macroH2A1.2 promotes KDM5A damage recruitment. GFP-KDM5A damage recruitment was performed as in A. Dotted white circles indicate damaged regions. Scale bars, 5 µm. (D) Quantification of C as in B. n > 10 cells per condition. P values were calculated using an unpaired Student’s t test for B and D (**, P < 0.01; ***, P < 0.001).