Figure S3.

KDM5A interacts with PAR chains. Related to Figs. 2 and 4. (A) Endogenous KDM5A interacts with PAR chains. Untreated or IR-treated U2OS cells were immunoprecipitated with KDM5A antibody and analyzed with the indicated antibodies. (B) PAR IP identifies increased KDM5A interactions following DNA damage. Experiment was performed as in Fig. 2 C. (C) Immunoprecipitated KDM5A from U2OS cells binds PAR. Experiment was performed as in Fig. 2 E. (D) Analysis of MBP-KDM5A fragments binding to PAR. 10 pM of H2A, BSA, MBP, and MBP-KDM5A fragments F1–F8 were spotted onto nitrocellulose membrane, and PAR-binding analysis was performed as in C. (E) GFP-KDM5B is not recruited to laser-induced DNA damage in either HCT116 WT or KDM5A-KO cells. Scale bars, 5 µm. Dotted white circles specify damaged region.