Figure S4.

Identification of a conserved putative coiled-coil domain unique to KDM5A. Related to Fig. 4. (A) KDM5A PID is intrinsically disordered. Diagram of KDM5A aligned with a disorder tendency plot (red graph). The disorder tendency of KDM5A was calculated using IUPred. Globular, structured domains in KDM5A are shown in light blue boxes. Magenta box indicates PID. (B) Alignment of human KDM5A aa 1408–1623 and the corresponding regions of human KDM5B and KDM5C. Amino acids are color coordinated as indicated in the legend. PAR-binding–predicted coiled-coil region is indicated by the magenta dotted line. Multiple sequence alignment was performed using Clustal Omega. (C) A multiple sequence alignment of Homo sapiens KDM5A aa 1496–1615 with Mus musculus, Rattus norvegicus, Pan troglodytes, Canis familiaris, Bos taurus, Gallus gallus, Equus caballus, Sus scrofa, Ovis aries, Felis catus, and Xenopus tropicalis. KDM5A exhibits high conservation in this C-terminal region. Colored amino acids are as in B. For B and C, asterisks denote fully conserved residues; colon indicates conservation between strongly similar residues; period indicates conservation between weakly similar residues.