FIGURE 1.

Effect of age, ACA, Rapa, and 17aE2 treatments on hepatic levels of several proteins regulated by CIT. (a) Representative Western blots for CIT‐regulated proteins: MGMT, NDRG1, TFAM, and Hsp70 in liver samples from young untreated (Y), old untreated (O), old treated with ACA or Rapa (O, early and late treatments). (b) Representative Western blots showing the effects of age and 17aE2 (early and late) treatments separated by sex (males = M, females = F) for the same CIT‐regulated proteins. (c) Bar graphs represent the mean ± SEM for each of the CIT‐regulated proteins in liver samples obtained from 16 young and 16 old mice plus at least 8 mice for each of the ACA and Rapa groups. Data for 17aE2 were obtained from 8 young and 8 old mice and a minimum of 4 for each male and female group. All values have been normalized to young control females as described in Methods section. The (*) indicates statistical significance (p < 0.05) in a t test between the indicated pair of groups