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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Clin Pharmacol Ther. 2020 May 23;108(3):671–680. doi: 10.1002/cpt.1847

Figure 3.

Figure 3.

Paclitaxel reduces the number of neurites in SH-SY5Y-derived neurons and this effect is exacerbated by inhibition of P-glycoprotein by verapamil. Fully differentiated SH-SY5Y cells were treated with the indicated concentrations of paclitaxel for 24 hours in the absence and presence of 5 μM and 50 μM verapamil. A) The cells were stained for F-actin and nuclei (DAPI) and representative images are shown. Exposure to 0.5 μM of paclitaxel causes minimal effects on neuronal networks compared to the 0.2% DMSO control, but concomitant treatment with 50 μM of the P-gp inhibitor verapamil causes increased neuronal toxicity of paclitaxel. Scale bar represents 200 μm. B) The number of neurites were quantified using ImageJ. At least five images from three separate differentiations were assessed. Verapamil (5 μM and 50 μM) significantly exacerbated the paclitaxel (0.1 μM and 0.5 μM) effect on number of neurites (p<0.001, ANOVA).