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Overexpression of circSKIL promotes the proliferation and mineralization of control cells. Control cells were transfected with either a circSKIL-overexpressing plasmid or an empty vector (NC). (A) Confirmation of successful circSKIL overexpression (n=3). (B) Cell proliferation was assessed by CCK-8 assay (n=3). (C) Representative images and (D) quantification of Alizarin Red S staining (n=3). *P<0.05 and **P<0.01 vs. NC. circ, circular RNA; NC, negative control; OD, optical density.
