Figure 4.

Downregulation of circSKIL inhibits the proliferation and mineralization of OPLL cells. OPLL cells were transfected with either a circSKIL-targeting siRNA or a negative control siRNA (si-NC). (A) Confirmation of successful circSKIL silencing (n=3). (B) Cell proliferation was assessed by CCK-8 assay (n=3). (C) Representative images and (D) quantification of Alizarin Red S staining (n=3). ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. si-NC. circ, circular RNA; OPLL, ossification of the posterior longitudinal ligament; si, small interfering; NC, negative control; OD, optical density.