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. 2021 May 13;22(1):761. doi: 10.3892/etm.2021.10193

Figure 5.

Figure 5

Downregulation of circSKIL suppresses osteogenic differentiation and inhibits the JNK/STAT3 pathway in OPLL cells. OPLL cells were transfected with either a circSKIL-targeting siRNA or a negative control siRNA (si-NC). (A) mRNA expression levels of RUNX2, ALP, COL I, OCN, and OPN (n=3). (B) Western blot analysis of the protein expression levels of RUNX2, ALP, COL1A1, OCN, and OPN (n=3). (C) Western blot analysis of the protein levels of p-JNK, JNK, p-STAT3, and STAT3 (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. si-NC. circ, circular RNA; OPLL, ossification of the posterior longitudinal ligament; si, small interfering; NC, negative control; RUNX2, runt-related transcription factor 2; ALP, alkaline phosphatase; COL I, collagen I; OCN, osteocalcin; OPN, osteopontin; COL1A1, collagen type I α 1 chain; p-, phosphorylated.