Figure 2.

Downregulation of RPLP1 inhibits the proliferation of Hep3b cells. (A) Reverse transcription-quantitative PCR analysis was performed to detect RPLP1 mRNA expression in Hep3b cells. (B) A Cell Counting Kit-8 assay was performed to assess cell viability. (C) A colony formation assay was performed to assess cell proliferation. (D) Immunofluorescence analysis was performed to detect Ki-67 expression. Scale bars, 50 µm. (E) Western blot analysis was performed to detect the protein expression levels of MCM and PCNA. ^**P<0.01, ^***P<0.001 vs. control. RPLP1, ribosomal protein LP1; MCM, minichromosome maintenance; PCNA, proliferating cell nuclear antigen; sh, short hairpin; NC, negative control.