Figure 4.

Downregulation of RPLP1 suppresses the migratory and invasive abilities of Hep3b cells. (A) Western blot analysis was performed to detect the protein expression levels of MMP-2, MMP-9 and TIMP-1. (B and C) Wound healing assay was performed to assess cell migration. Scale bars, 100 µm. (D and E) Transwell assay was performed to assess cell invasion. Scale bars, 100 µm. ^**P<0.01 and ^***P<0.001 vs. control. RPLP1, ribosomal protein LP1; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of matrix metalloproteinase-1; sh, short hairpin; NC, negative control.