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. 2021 Apr 29;67(9):1249–1258. doi: 10.1093/clinchem/hvab069

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Fig. 3. — SARS-CoV-2 antibody avidity assay characterization with model COVID-19 purified antibodies. (A), Dissociation curve measurement of COVID-19–negative human serum spiked with 5 different COVID-19 antibodies. Signal_0/Signal_t is the normalized fluorescent signal on the y axis. (B), Relative dR measurement at varying levels of Signal_0. The COVID-19–negative human serum was spiked with 7 COVID-19 antibodies at different levels and measured. (C), Correlation between the SARS-CoV-2 avidity assay (dR measurement) and the BLI measurement (k_off). Correlation between the 2 assays was assessed by the Spearman correlation coefficient.