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Relationship between β-actin and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection probability. Binomial logistic regression relating SARS-CoV-2 detection to β-actin cycle threshold (Ct) value reveals a negative association, with high β-actin Ct (ie, low quality) nasopharyngeal specimens less likely to detect SARS-CoV-2. The absolute probability of SARS-CoV-2 detection is presented in relation to the observed range of β-actin Ct values.
