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. 2021 May 19;22:256. doi: 10.1186/s12859-021-04180-x

Fig. 1.

Fig. 1

Overview of methods to determine COI nuMT characteristics and test methods for nuMT removal. Dataflow for our a artificial DNA barcode dataset, b perturbed community datasets, and c real freshwater COI metabarcode dataset. Abbreviations: BOLD = Barcode of Life Data System; COI = cytochrome c oxidase subunit I mtDNA gene; HMM = hidden Markov model; NCBI = National Centre for Biotechnology Information; nt = nucleotide; ORF = open reading frame