Table 2.
Growth conditions for conidia production in Pyrenophora sp. Columns differentiate growth phases and row describe parameters. All listed conditions were significantly associated with increased conidia production and purity
| Parameters | Vegetative | Conidiophore | Conidia | Harvest |
|---|---|---|---|---|
| Duration | 5–7 days | 24 h | 18 h | – |
| Light Source | White | N-UV | Darkness | – |
| Light Cycle | 12 h Light/Dark | Continuous | Continuous | – |
| Temperature | 22–24 °C | 22–24 °C | 15 °C | – |
| Plate Lid | Sealed | Removed | Loose | – |
| Media | V8-PDA | V8-PDA | – | 0.02% Tween 20 |
| Plug Location | Total Mycelia | – | – | – |
| Wounding Method | 200 µl Tip End | – | – | – |
| Extraction | – | – | – | Small brush Excess agar removed |