Table 3.
The optimized sporulation protocol tested on Ptr strains and Pyrenophora species resulting in elevated conidia numbers
| Species | Isolate | Race | Year Isolated | Location | Crop | Average Spores Per Plate | Traditional Method | Increase |
|---|---|---|---|---|---|---|---|---|
| Ptr | M4 | 1 (ToxA and ToxC) | 2009 | Meckering (WA) | Wheat | 3.00E+06 | 2.00E+03 | 1500 X |
| Ptr | DW5 | 5 (ToxB) | 1998 | North Dakota (USA) | Wheat | 1.79E+06 | 1.50E+03 | 1194 X |
| Ptr | 15 FRG 233 | 1 (ToxA and ToxC) | 2015 | Esperance (WA) | Wheat | 1.37E+06 | ||
| 15 FRG 239 | 1 (ToxA and ToxC) | 2015 | Yarrawonga (VIC) | Wheat | 3.30E+06 | |||
| 15 FRG 022 | 1 (ToxA and ToxC) | 2015 | Dandaragan (WA) | Barley | 1.75E+06 | |||
| 17 FRG 118 | 1 (ToxA and ToxC) | 2017 | Wagin (WA) | Wheat | 2.47E+06 | |||
| 15 FRG 230 | 1 (ToxA and ToxC) | 2015 | Cunderdin (WA) | Wheat | 3.00E+06 | |||
| 17 FRG 135 | 1 (ToxA and ToxC) | 2017 | Cowra (NSW) | Wheat | 2.64E+06 | |||
| 16 FRG 175 | 1 (ToxA and ToxC) | 2016 | Wunkar (SA) | Wheat | 1.15E+06 | |||
| Ptt | W1-1 | "Beecher avirulent" | 2009 | Wongan Hills (WA) | Barley | 1.00E+06 | 1.00E+03 | 1000 X |
| Ptm | SG1 | unknown | 1996 | Badgingarra (WA) | Barley | 8.10E+05 | 5.00E+03 | 162 X |