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. 2021 Apr 16;12:678999. doi: 10.3389/fimmu.2021.678999

Figure 6.

Figure 6

Kynurenine induces PD-1 expression on CD8+ T cells in an AHR-dependent manner. (A) UCSC Genome Browser plot of open chromatin accessible regions in two DNA regulatory elements in the PD1 gene identified by ATAC-seq analysis of CD8+ T cells, from WT C57BL/6 mice, activated by anti-CD3/CD28 and treated with KYN (50µM) or TCDD (10nM) for 2 and 48 hrs. (B) CHIP-qPCR Pdcd1 analysis in anti-CD3/CD28 bead-activated and KYN (50µM) treated CD8+ T cells for 2 hours from spleens of WT C57BL/6 mice with AHR antibody and pdcd1 promoter primers. (C) Anti-CD3/CD28 activated CD8+ T cells from spleens of WT C57BL/6 mice were treated with KYN (50µM) and CH223191(AHR antagonist) (3μM) for 6 days. Cyp1a1 and (D) PD-1 mRNA was determined. **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t test (B–D). The data represent means ± SEM of three independent experiments performed in triplicate (B–D).