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. 2021 May 17;9:e11336. doi: 10.7717/peerj.11336

Table 2. Cytogenetic results.

Species Chromosome number Inferred 2n Populations
Clade A
C. lancea 2n = 44II in MI 88 South Africa, Western Cape, Table Mountain. IX-2009. 4544MM (BOL).
C. multispiculata 2n = 31II in MI 62 Two populations: South Africa, KwaZulu-Natal, Cathedral Peak Nature Reserve, 1,711 m, 11-XI-2012, 53EMS12(1) (UPOS). Ibidem, 2,287 m, 58EMS12 (UPOS).
2n = 1III+29II+1I in MI 62 South Africa, KwaZulu-Natal, Cathedral Peak Natural Reserve, 1,711 m, 11-XI-2012, 53EMS12(2) (UPOS).
2n = ca. 62 in premeiotic mitosis ca. 62 South Africa, KwaZulu-Natal, Mlambonja Wilderness area, 1,700 m, 17-XI-2010, 129SMB10 (UPOS).
C. schweickerdtii 2n = 30II in MI 60 South Africa, KwaZulu-Natal, Cathedral Peak Nature Reserve, 2,287 m, 11-XI-2012, 57EMS12 (UPOS).
Clade B
C. burkei 2n = 33II in MI 66 South Africa, KwaZulu-Natal, Garden Castle Nature Reserve, 1,803 m, 16-XI-2012, 83EMS12 (UPOS).
2n = 35II in MI 70 South Africa, KwaZulu-Natal, road to Sani Pass, 2,258 m, 21-XI-2010, 158SMB10 (UPOS).
2n = 36II in MI 72 South Africa, Western Cape, W of Teepunt, Nuweveldberge, 1,844 m, 4-III-2008, Clark 51 (UPOS).
C. killickii 2n = 1III+34II in MI 71 South Africa, KwaZulu-Natal, Garden Castel Forest Reserve, 2,286 m, 19-XI-2010, 134SMB10 (UPOS).
2n = 36II in MI 72 South Africa, KwaZulu-Natal, Monk’s Cowl Nature Reserve, 1,650 m, 14-XII-2008, 77ML08 (UPOS-3620).
n = 36 in Pollen Grain Mitosis 72 South Africa, KwaZulu-Natal, Bushman’s Nek Area, 1,846 m, 15-XI-2012, 72EMS12 (UPOS).
Clade C
C. capensis n = 36 in Pollen Grain Mitosis 72 South Africa, Western Cape, Overberg area (BOL).
C. sciocapensis n = 34 in Pollen Grain Mitosis 68 South Africa, Western Cape, Kannaland, Towerkop Nature Reserve, 906–968 m, 12-X-2017, 142JMC17bis (UPOS).
Clade D
C. perdensa 2n = 15II in MI 30 South Africa, KwaZulu-Natal, Bushman’s Nek, 1,804 m, 15-IX-2012, 74EMS12 (two individuals; UPOS). Ibidem, 1,850 m, 20-XI-2010, 152SMB10 (UPOS).
n = 15 in Pollen Grain Mitosis 30 South Africa, KwaZulu-Natal, Bushman’s Nek, 1,804 m, 15-IX-2012, 74EMS12 (UPOS).
C. spartea n = 12 in Pollen Grain Mitosis 24 South Africa, KwaZulu-Natal, Bushman’s Nek Natural Reserve, 1,800 m, 20-XI-2010, 154SMB10 (UPOS).
n = 13 in Pollen Grain Mitosis 26 South Africa, Western Cape, Mossel Bay, 325 m, 14-X-2017, 183JMC17 (UPOS).
C. dregeana 2n = 16II in MI 32 South Africa, Free State, Scheepershoek, XI-2009, M. Muasya 4947MM (BOL).
n = 17 in Pollen Grain Mitosis 34 South Africa, KwaZulu-Natal, Bushman’s Nek, 1,779 m, 15-XI-2012, 70EMS12a (UPOS).
2n = 17II in MI 34 South Africa, KwaZulu-Natal, near Boston, 1,450 m, 8-X-2009, 4900bMM (UPOS).
C. dregeana x C. spartea n = 13, 14 in Pollen Grain Mitosis 27 Two populations: South Africa, KwaZulu-Natal, Bushman’s Nek, 1,779 m, 15-XI-2012, 70EMS12d (UPOS). South Africa, KwaZulu-Natal, Garden Castle Nature Reserve, 1,803 m, 16-XI-2012, 84EMS12 (UPOS).
n = 12, 13, 14 in Pollen Grain Mitosis 26 South Africa, KwaZulu-Natal, Bushman’s Nek, 1,800 m, 20-XI-2010, 155SMB10 (UPOS).
n = 14, 15, 16 in Pollen Grain Mitosis 30 South Africa, KwaZulu-Natal, Bushman’s Nek, 1,850 m, 20-XI-2010, 153SMB10 (UPOS).
C. esenbeckiana 2n = 13II in MI 26 Three populations: Kenya, Laikipia county, Ndaragwa forest, 2,300 m, 25-VII-2007, 70UPO-K (UPOS). South Africa, Western Cape, Table Mountain, 17-II-2012, 6459MM (BOL). South Africa, Brown Hooded Kingfisher path, 14-X-2017.
Clade E
C. badilloi n = 17 in Pollen Grain Mitosis 34 South Africa, KwaZulu-Natal, Garden Castle, 1,860 m, 16-IX-2012, 76EMS12a (UPOS).
n = 18 in Pollen Grain Mitosis 36 Ibidem, 76EMS12b (UPOS).
C. ludwigii 2n = 17II in MI 34 Three populations: South Africa, KwaZulu-Natal, Garden Castle Nature Reserve, 1,810 m, 16-XII-2008, 106ML08 (UPOS). Ibidem, road to Sani Pass, 2,225 m, 21-XI-2010, 162SMB10 (UPOS). Ibidem, 159SMB10 (UPOS).
n = 17 in Pollen Grain Mitosis 34 Three populations: South Africa, KwaZulu-Natal, Nganang river, Drakensberg Gardens hotel, 1,794 m, 9-XI-2009, 4922MM (UPOS). Ibidem, road to Sani Pass, 2,225 m, 21-XI-2010, 162SMB10 (UPOS). Ibidem, 159SMB10 (UPOS).
C. bolusii 2n = 18II in MI 36 Two populations: South Africa, Free State, Golden Gate Highlands National Park, 1,967 m, 15-I-2010, 42ML10 (UPOS). Ibidem, Bushman’s Nek Nature Reserve, 1,779 m, 15-XI-2012, 70EMS12b (UPOS).
n = 18 in Pollen Grain Mitosis 36 Two populations: South Africa, Free State, Golden Gate Highlands National Park, 1,800-2,000 m, 13-XII-2008, 143SMB08 (UPOS). Ibidem, KwaZulu-Natal, Garden Castle Nature Reserve, 1,803 m, 16-XI-2012, 77EMS12 (UPOS). Ibidem, 2,267 m, 19-XI-2010, 141SMB10 (UPOS).
C. pseudorufa 2n = 32II in MI 64 South Africa, KwaZulu-Natal, Cathedral Peak Nature Reserve, 2,307 m, 11-XI-2012, 59EMS12 (UPOS).

Note:

Species names, chromosome number (in Metaphase I or Pollen Grain Mitosis), inferred diploid chromosome number and information of population of origin are indicated. Populations data include country, province, locality, altitude, collection code (in bold) and the herbarium where the witness voucher is preserved. The symbols I, II and III represent univalent, bivalent and trivalent chromosome association, respectively.