Figure 2.
Distribution of Binding Affinities Employed in Bayesian fup Estimations. To jointly analyze the data from the RED assay conducted at three protein concentrations, a model for binding to protein with an dissociation constant (i.e., binding affinity) must be assumed. Although the specific binding protein (for example, hemoglobin) and the stoichiometry are unknown, this number provides a rough estimate for each chemical of how much that chemical would be expected to be impacted by the presence of plasma binding.